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You are here: Home Administration Chemistry & Biochemistry Department Events 2019 January Biochemistry Seminar: Thomas Walz, "An Experimental Look at Hydrophobic Mismatch"

Biochemistry Seminar: Thomas Walz, "An Experimental Look at Hydrophobic Mismatch"

Thomas Walz, Professor, Biochemistry, Biophysics, Chemical Biology, and Structural Biology, Rockefeller University, NY, "An Experimental Look at Hydrophobic Mismatch"
When Jan 30, 2019
from 12:00 PM to 01:00 PM
Where CUNY ASRC Main Auditorium
Contact Name
Contact Phone 212-650-8803
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ABSTRACT

Hydrophobic mismatch, the difference between the hydrophobic thickness of a membrane protein and its surrounding lipid bilayer, is thought to be a general mechanism by which the membrane environment can affect the function of membrane proteins or their organization within a membrane.  To experimentally test this notion, we used cryo-EM to study the structure of membrane proteins in the context of lipid bilayers.  Structures obtained by electron crystallography of the water channel aquaporin-0 (AQP0) reconstituted into two-dimensional (2D) crystals with lipids that form bilayers of different thickness showed no evidence that the lipid bilayer affects the structure of AQP0.  However, structures of AQP0 in raft lipids suggest principles that may underlie AQP0 array formation and potentially raft formation in general, for which hydrophobic mismatch may be a contributing factor.  For the mechanosensitive channel MscS reconstituted into nanodiscs with different lipids, structures obtained by single-particle cryo-EM revealed that extreme hydrophobic mismatch can open the channel, but it does not appear to be the mechanism that regulates MscS under physiological conditions.

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