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You are here: Home Administration Chemistry & Biochemistry Department Events 2019 April Biochemistry Seminar: Kalina Hristova, "The EphA2 receptors: dimerization, structure, and function"

Biochemistry Seminar: Kalina Hristova, "The EphA2 receptors: dimerization, structure, and function"

Kalina Hristova, Professor, Materials Science and Engineering, Johns Hopkins Univ, MD, "The EphA2 receptors: dimerization, structure, and function"
When Apr 10, 2019
from 12:00 PM to 01:00 PM
Where CUNY ASRC Main Auditorium
Contact Name
Contact Phone 212-650-8803
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ABSTRACT

The EphA2 receptor tyrosine kinase is capable of activating multiple diverse signaling pathways with roles in processes such as tissue homeostasis and cancer. EphA2 is known to form activated oligomers in the presence of ephrin-A ligands. We characterized the lateral interactions between full-length EphA2 molecules in the plasma membrane in the presence of three types of ligands (dimeric ephrinA1-Fc, monomeric ephrinA1, and an engineered peptide ligand) as well as in the absence of ligand, using a quantitative FRET technique. The data show that EphA2 forms higher-order oligomers and two different types of dimers that all lead to increased EphA2 tyrosine phosphorylation, which is indicative of increased kinase-dependent signaling. We find that different ligands stabilize conformationally distinct oligomers that are assembled through two different interfaces. Our results suggest that these different oligomeric assemblies could have distinct signaling properties, contributing to the diverse activities of the EphA2 receptor.

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