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LSM 710 - Confocal Scanning Microscope

Location and contact information

Fees

External federal users - $85/h
CCNY users - $20/h

Long-running experiments qualify for a 50% discount.  See Add. I in the facility usage rules for more info.

In addition, the facility implements a cap of $1500/month for all usage (combined) of LSM 710, LSM 800, LSM 880, and Nikon TiE.

Facility Images

 

Confocal System Microscope stand

 

Background Information

Confocal laser scanning microscopy (CLSM or LSCM) is a technique for obtaining high-resolution optical images.[1] The key feature of confocal microscopy is its ability to produce in-focus images of thick specimens, a process known as optical sectioning. Images are acquired point-by-point and reconstructed with a computer, allowing three-dimensional reconstructions of topologically-complex objects. The principle of confocal microscopy was originally patented by Marvin Minsky in 1957,[2] but it took another thirty years and the development of lasers for CLSM to become a standard technique toward the end of the 1980s. (source: Wikipedia)

Documents

LSM 710 Manual (PDF)

Introduction to Confocal Microscopy (PDF)

Confocal Microscopy Guide (PDF)

Equipment Specifications

Please note: More detailed information about the system can be obtained from our staff.

Services Offered:

Confocal Fluorescent Imaging in 4 channels including Zeiss Meta spectral detector. 
Brightfield and DIC Imaging (using a transmitted light detector)
User Training.
Data analysis and post-processing.

Excitation sources:

Diode Laser (405 nm) - 30mW
Ar Laser (458/488/514 nm) - 25mW
DPSS 561 - 15mW
Hene Laser (594nm) - 2mW
Hene Laser (633nm) - 5mW
X-Cite 120 Series Illumination system

Detectors:

Zeiss Meta (QUASAR) detector with step size 3.2 - 38.9 nm.

Stand:

Observer.Z1 inverted with motorized focusing drive, fully motorized stage, definite focus system.

FL Filters (visual observation):

Combination of filter sets allowing for simultaneous use with dyes excited in:

Blue spectral range - 458/488nm (GFP,CFP,LUCIFER YELLOW, BCECF, FITC, CY2, ACRIDINE ORANGE)
Green Spectral Range - 543nm (DSRED,RHODAMINE (TRITC), CY3, TEXAS RED, PROPIDIUM IODIDE
Red Spectral Range - 633nm (CY5,DID,ALEXA FLOUR 633)
Violet Spectral Rnage - 405nm (DAPI,HOECHST,AMCA,BFP)

Optics:

Eyepiece PL 10x/23 focusing
EC Plan-Neofluar 10x/0.3 NA
Plan Apo 20x/0.8NA DRY DIC
C-Apochromat 40x/1.2 Water Korr
EC plan neofluar 40x/1.3NA OIL DIC
Plan apo 63x/1.4NA OIL DIC
DIC prism II
DIC prism III
analyzer D/DIC
polarizer D ROT removable

Software:

Zeiss Zen 2011 64 bit Black Edition

Additional Resources:


Dyes: https://www.chroma.com/spectra-viewer

Fluorescence: http://www.thermofisher.com/us/en/home/support/tutorials.html#vid1

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