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LSM 880 - Confocal Scanning Microscope

Facility News

  1. New rates effective Feb 1st, 2016 (more)
  2. Weekend users or those wishing to use the equipment before 10 am and after 6pm MUST be able to drive the microscope without ANY assistance.

Location and contact information

Fees

External users - $87/h
CCNY users - $30/h

Long-running experiments qualify for a 50% discount.  See Add. I in the facility usage rules for more info.

Facility Images

 

Confocal System Microscope stand

 

Background Information

Confocal laser scanning microscopy (CLSM or LSCM) is a technique for obtaining high-resolution optical images.[1] The key feature of confocal microscopy is its ability to produce in-focus images of thick specimens, a process known as optical sectioning. Images are acquired point-by-point and reconstructed with a computer, allowing three-dimensional reconstructions of topologically-complex objects. The principle of confocal microscopy was originally patented by Marvin Minsky in 1957,[2] but it took another thirty years and the development of lasers for CLSM to become a standard technique toward the end of the 1980s. (source: Wikipedia)

Documents

Introduction to Confocal Microscopy (PDF)

Confocal Microscopy Guide (PDF)

AiryScan guide (PDF)

Equipment Specifications

Stand:
  • Axio Observe.Z1 motorized basic stage.
  • Scanning stage 130x100mm step (surface 325x144mm). Resolution 0.1 um.
Excitation sources:
  • NLO Ar 561nm 633nm
  • HeNe 594nm
  • Diode 405nm
  • X-Cite 120 UV illumination system
Detectors:
  • Spectral Detection 3 ch with one GaAsP
  • AiryScan Module
  • Transmitted light detector T-MPT
Optics:
  • EC Plan-Neofluar 5x/0.16 WD=18.5
  • Plan-Apochromat 10x/0.45 WD=2.0
  • Plan-Apochromat 20x/0.8 WD=0.55
  • Plan-APO 40x/1.4 Oil DIC (UV) VIS-IR
  • Plan-Apochromat 63x/1.40 Oil DIC
  • C-Apochromat 40x/1.2 W Korr. FCS
  • Optics fully supporting DIC/PC imaging.
Software:
  • Zen 2
Additional Accessories:
  • Incubator PM 2000 RST with CO2, heating, humidity control with an objective heater.

Additional Resources:

Dyes: https://www.chroma.com/spectra-viewer

LSM880 Airyscan: https://www.youtube.com/watch?v=sGTNBXq8omg&t=1021s

Fluorescence: http://www.thermofisher.com/us/en/home/support/tutorials.html#vid1