500 MHz Nuclear Magnetic Resonance - NMR
Location and contact information
- Location: CCNY, Marshak 1201
- Facility Manager: Dr. Padmanava Pradhan
- Phone: 212 650 8697/8266
- Hours: Please call
- Scheduling calendar: click here
- External website: http://www.sci.ccny.cuny.edu/nmr/nmr_ccny.htm
The Varian (Agilent) Unity Invova-500 NMR (Nuclear Magnetic Resonance) spectrometer of the City College of the CUNY is located in the Plaza level of Marshak science building. The magnet has a field strength of 11.74 T (1H frequency of 500 MHz). This instrument performs a wide variety of multinuclear (1H, 13C, 31P, 11B and 15N) including 2D NMR experiments at variable temperature for structural identification of synthetic compounds. It is used extensively elucidation of structure and stereochemistry of organic compounds and solution conformations of DNA adducts. Prof. Lakshman, Prof. Biscoe, Prof. Zajc and Prof. Koder are the major user of this spectrometer.
1H, 13C, 15N, 31P, 11B, other nuclei
Solvent Suppression, 1D NOE, DEPT
1D with variable temperature (-60º to 100º C)
All normal and gradient selected 2D experiments including gCOSY, TOCSY, HSQC, gHMQC, HMBC, ROESY, NOESY etc.
5mm PFG Switchable (Standard Probe) X/H
Tunable to X nuclei from 31P (202 MHz) to 15N (50 MHz)
Ideal for detection of broadband nuclei
Used for most 2D experiments including COSY, TOCSY, HSQC, HMBC, ROESY, NOESY
Variable Temperature (-60 º to 100 º C)
M. K. Lakshman, A. Deb, R. R. Chamala, P. Pradhan, R. Pratap. Direct arylation of 6 phenylpurine and 6-arylpurine nucleosides by ruthenium-catalyzed C-H bond activation, Angewandte Chemie, International Edition 2011, 50, 11400-11404. DOI: 10.1002/anie.20110403
R. Kumar, P. Pradhan and B. Zajc. Facile synthesis of 4-vinyl- and 4-fluorovinyl-1, 2, 3-triazoles via bifunctional “click-olefination” reagents. (2011) Chem. Commun. 47, 3891-3893.
V.S. Balachandran, S. R. Jadhav, P. Pradhan, S. De Carlo and G. John. Adhesive Vesicles through Adaptive Response of a Biobased Surfactant. (2010). Angew. Chem. Int. Ed. 4, 9509.
Synthesis of N6,N6-Dialkyladenine Nucleosides Using Hexaalkylphosphorus Triamides Produced in Situ. M. K. Lakshman A. Choudhury, S. Bae, E. Rochttis, P. Pradhan, A. Kumar (2009) Eur. J. Org. Chem. 152-159.
Palladium-Catalyzed Synthesis of Nucleoside Adducts from Bay and Fjord Region Diol Epoxides. E. Champeil, P. Pradhan and M. K. Lakshman (2007) J. Org. Chem. 72(14) pp 5035 – 5045.
Highly diastereoselective synthesis of nucleoside adducts from the carcinogenic benzo[a]pyrene diol epoxide and a computational analysis. M. K. Lakshman, J. C. Keeler, F. N. Ngassa, J. H. Hilmer, P. Pradhan, B. Zajc and K. A. Thomasson (2007) J. Am. Chem. Soc., 129, 68-76.
Voltage gating and anions, especially phosphate: A model peptide. P. Pradhan, R. Ghose and M. E. Green (2005) Biochim. Biophys Acta. (2005) 1717, 97-103.
Synthesis and Reactions of 2-Chloro- and 2-Tosyloxy-2'-deoxyinosine Derivatives. N. Pottabathini, S. Bae, P. Pradhan, H. Hanh, H. Mah and M. Lakshman (2005), J. Org. Chem. 70(18); 7188-7195.
Mild and Room Temperature C-C bond forming reactions of nucleoside C-6 arylsulphonates, M. K. Lakshman, P. Gunda and P. Pradhan. (2005) J. Org. Chem. 70 (25), 10329-10335
Ghosh, A. K.; Zajc, B.: Fluorinated 1-Phenyl-1H-tetrazol-5-yl Sulfone Derivatives as General Reagents for Fluoroalkylidene Synthesis, J. Org. Chem. 2009, 74, 8531–8540.
Zajc, B.; Kumar, R.: Synthesis of Fluoroolefins via Julia-Kocienski Olefination, Synthesis 2010, 42, 1822-1836.