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600 MHz Solid State NMR

Location and Contact Information

  • Hours: Open 24/7 to all qualified users.
  • Scheduling calendar: click here

Fees

The following user fees are effective as of 03/01/17

Federal Users - $9.50/h
Non-federal Users - $19.00/h
CCNY subsidized - $4.00/h

Images

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About the instrument

Description

The Varian VNMRS 600 MHz Solids NMR spectrometer is a mixed-use instrument  that is capable of  carrying out NMR experiments in solution, semi-solid, as well as solid states.  The main focus, however, is in solid state research.

The instrument operates with an actively-shielded 14.7 Tesla superconducting magnet.  It has a small 5-Gauss stray field boundary with a radius of 6 ft.  The proton NMR frequency is at 600 MHz.

The instrument contains 4 radio frequency (RF) channels, and is capable of carrying out sophisticated multi-nuclear, multi-dimensional experiments in structural biology, both in solution and solid states.  Two of the channels are equipped with kilowatt amplifiers and the other two with 300 watt amplifiers to deliver sufficient power to the probe for demanding solid state experiments.

Most solid state experiments are carried out under magic-angle-spinning (MAS) conditions.  In solids, dipolar interactions broaden the linewidth such that the spectral feature cannot be discerned.  Spinning at 54.7 deg with respect to the magnetic field averages out dipolar interactions and peaks become sharp.  Our fastest spinning probe is capable of spinning up to 40 kHz. (See Probes section.)

Our solid state NMR spectrometer is a sophisticated instrument that requires extensive training to operate.  Clearly it cannot be a walk-up experience. The applications, however, are wide-ranging.  Both of our solids probes (see below) are of HXY-type, where two of the channels are broadbanded from P31 to N15.  Current research interests include structural biology studies of isotopically labeled (C13, N15, and H2) proteins, natural-abundance as well as isotopically labeled plant and fungal materials, P31 studies of lipids and protein phosphorylation, and B11 studies of energy storage materials.

Services

  • Multi-dimensional, multi-nuclear solid state NMR research on proteins
  • Multi-dimensional, multi-nuclear solution state NMR research on proteins
  • Solid state NMR research on materials, such as natural or synthetic polymers, and energy storage materials
  • High sensitivity solution state NMR research with broadbanded nuclei such as B11, P31, Si29, etc.
  • Study of Semi-solid materials such as biological tissues or swollen polymers

Probes

  1. 3.2 mm BioMAS Probe: A 3 channel (HXY) MAS probe that spins up to 25 kHz.  It has a scroll coil that is designed to minimize electric field heating in samples that have high salt content, with some compromise in sensitivity.  It is a workhorse for multidimensional, multinuclear solid state protein research.
  2. 1.6 mm FastMAS Probe: A 3 channel (HXY) MAS probe that spins up to 40 kHz.  The probe could offer higher resolution with reduced dipolar coupling because of the high spinning speed.  It is optimal for J-directed polarization transfer experiments.  Despite the small size of sample rotor (1.6 mm), the probe has good sensitivity.
  3. 4 mm gHX nano Probe: A 2 channel (HX) probe that spins at magic angle up to 3 kHz and equipped with a z-pulsed field gradient.  It can run most of the solution state NMR experiments on solution or semi-solid samples, such as tissue samples or swollen polymer samples.
  4. 5 mm IDQG Probe: A 4 channel (H1, C13, N15, and P31) probe that is equipped with z-pulsed field gradient as well as deuterium decoupling capabilities.  It is designed for multidimensional, multinuclear protein research in solution.
  5. 5 mm Dual Broadband Probe: A 2 channel (H1/F19, X) probe with X nuclei in the inner coil to provide high X-nuclei sentivity.  It is a versatile probe for a wide range of nuclei in solution

Recent Publications

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