Biophysical Chemistry (NMR)
From bacteria to humans, the life-cycle of a species depends on an delicately-balanced network of protein-protein interactions in space and time. We investigate a small subset of these interactions using a host of biophysical and biochemical techniques including solution-state nuclear magnetic resonance (NMR) methodology and recently, X-Ray crystallography.
Our current research topics include:
Intracellular signalling involving the Src-family kinases.
Structure and organization of the RNA polymerase complex in cystoviruses.
Development of novel NMR methods to study correlated dynamics on multiple timescales in proteins.
Development of NMR methods to obtain structural constraints in large proteins/complexes.
Del Rio A, Dutta K, Chavez J, Ubarretxena-Belandia I and Ghose R. (2007) Solution Structure and Dynamics of the N-terminal Cytosolic Domain of Rhomboid Intramembrane Protease from Pseudomonas aeruginosa: Insights into a Functional Role in Intramembrane Proteolysis. J. Mol. Biol. 365, 109.
Del Rio A, Anand A and Ghose R (2006) Detection of Correlated Dynamics on Multiple Timescales by Measurement of the Differential Relaxation of Zero- and Double-Quantum Coherences Involving Sidechain Methyl Groups in Proteins. J. Magn. Reson. 180, 1.
Dutta K, Shi HH, Cruz-Chu ER, Kami K and Ghose R (2004) Dynamic influences on a high-affinity, high-specificity interaction involving the C-terminal SH3 domain of p67phox. Biochemistry 43, 8094.
Majumdar A and Ghose R (2004) Probing slow backbone dynamics in proteins using TROSY-based experiments to detect cross-correlated time-modulation of isotropic chemical shifts. J. Biomol. NMR 28, 213.
Ghose R, Shekhtman A, Goger MJ, Ji H and Cowburn D (2001) A novel, specific interaction involving the Csk SH3 domain and its natural ligand. Nature Struct. Biol. 8, 998.
Ghose R, Fushman D and Cowburn D (2001) Determination of the Rotational Diffusion Tensor of Macromolecules in Solution from NMR Relaxation Data with a Combination of Exact and Approximate Methods. J. Magn. Reson. 149, 204.
Ghose R. (2000) Average Liouvillian Theory in Nuclear Magnetic Resonance - Principles, Properties and Applications. Concepts Magn. Reson. 12, 152.