Cancer & Malignancy
- B.A. University of Michigan
- Ph.D. University of Pennsylvania
The goal of our research is to understand the process by which human epithelial cells come to acquire the properties of tumor cells derived from malignant neoplasms. For this purpose this laboratory has utilized a model system based upon infection of cultures of normal human epidermal cells by the oncogenic virus, SV40. Three major aspects of transformation in this system are currently under investigation: 1) alteration in the structure and expression of endogenous oncogene sequences caused by SV40 infection, 2) alteration in the structure and expression of the SV40 virus transforming genes occurring after long term infection, and 3) SV40-induced expression of normally silent intermediate filament genes as a model of altered gene expression in transformed epithelial cells.
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Chen,W., Ruml, T. and Steinberg, M.L.: Characterization of a 1.8 kb DNA segment located upstream from the human Ha-ras protooncogene and possibly regulating its function. Gene, 155(2):253-256, 1995.
Guangtian Chen, G.T, Baez, J. and Steinberg, M.: integration of the SV40 promoter/enhancer sequences in an anchorage independent clonal subline of SV40-infected human keratinocytes. Gene, 183:41-45, 1996.
Steinberg, M. Immortalization of Human Epidermal Keratinocytes by SV40. in Culture of Immortalized Cells. Chapter 5, pp. 95-120, Ian Freshney ed., Wiley-Liss, New York, 1996
Vazquez, R. and Steinberg, M.L.: Enhancement of Genomic DNA Templates for Polymerase Chain Reactions by Partial Restriction Digestion. Biotechniques 26:91-95, 1999.
Steinberg, M., Guyden, J., Calhoun, D., Coico, R. and Staiaino-Coico, L. "Recombinant DNA Technology: Concepts and Biomedical Applications", Prentice Hall, Englewood Cliffs, New Jersey Inc., 1993
"The Facts on File Dictionary of Biotechnology and Genetic Engineering", Facts on File, Inc., New York, NY, contracted publication date: April, 1993