160 Convent Avenue
New York , NY 10031
New York , NY 10031
Email: saleques [ AT ] sci [ DOT ] ccny [ DOT ] cuny [ DOT ] eduOffice Phone: (212) 650-8538
Hematopoiesis, the biology of blood cell generation is an intricate and complex process that is vital for the health and survival of all mammals including human beings. Accordingly, any malfunctions in this process often lead to life threatening diseases such as anemias, leukemias and lymphomas. An investigation of the molecular mechanisms involved in producing normal blood cells is therefore essential for understanding the onset and progression of many hematopoietic diseases.
Our research focuses on the interplay between transcription factors and their co-regulators (genetic factors) and the chromatin alterations they catalyze (epigenetic processes) in orchestrating gene expression patterns that in turn program the fate and function of hematopoietic stem and progenitor cells. To address these issues, we utilize a combination of genetic, biochemical and molecular biological approaches including tissue culture manipulation of human and mouse cells in vitro and genetic manipulation of mice in vivo.
1. September 2008-present: Assistant Professor of Biology, City College of New York.
2. 2007- 2008: Senior research scientist (equivalent to research assistant professor) in the laboratory of Dr. Malcolm Moore at Memorial Sloan Kettering Cancer Center.
3. 1999–2007: post-doctoral fellow in Hematology and Oncology in the laboratory of Dr. Stuart H. Orkin at Children’s Hospital Boston and Harvard Medical School.
1990: Secured first position in the M. Sc. program in Biochemistry of Calcutta University.
1999-2003: Howard Hughes Medical Institute fellow.
2003–2006: Leukemia and Lymphoma Society Special Fellow.
Publications relevant to the proposed research:
1. Saleque, S., Kim, J., Rooke, H.M., and Orkin, S.H. 2007. Epigenetic regulation of hematopoietic differentiation by Gfi-1 and Gfi-1b is mediated by the cofactors CoREST and LSD1. Molecular Cell. 27(4): 562-572. http://www.cell.com/molecular-cell/abstract/S1097-2765(07)00483-2
2. Hock, H., Hamblen, M.J., Rooke, H.M., Schindler, J.W., Saleque, S., Fujiwara, Y., Orkin, S.H. 2004. Gfi-1 restricts proliferation and preserves functional integrity of haematopoietic stem cells. Nature.431: 1002-07. http://www.nature.com/nature/journal/v431/n7011/full/nature02994.html
3. Saleque S., Cameron, S. and Orkin,. S.H. 2002. The zinc-finger protooncogene Gfi-1b is essential for development of the erythroid and megakaryocytic lineages. Genes and Development. 16: 301-306. http://genesdev.cshlp.org/content/16/3/301.full.pdf
1. Zhou, J., Saleque, S., Ermakova, O., Sepulveda, M. A., Yang, Q., Eckhardt, L. A., Schildkraut, C. L., and Birshtein, B. K. 2005. Changes in replication, nuclear location, and expression of the Igh locus after fusion of a pre-B cell line with a T cell line. J. Immunol. 175; 2317-20.
2. Gordon, S. J., Saleque, S. and Birshtein, B.K. 2003. Yin Yang 1 (YY1) binding to the 3’ IgH enhancer is induced by class switching and cell cycle progression. J. Immunol. 170: 5549-5557.
3. Saleque, S., Singh, M. and Birshtein, B.K. 1999. IgH expression and class switching in vitro from an allele lacking the 3' enhancers DNase I hypersensitive sites hs3A and hs1,2. J. Immunol. 162 (5): 2791-2803.
4. Saleque, S., Singh, M., Little, R.D., Giannini, S.L., Michaelson, J.S. and Birshtein, B. K. 1997. Dyad symmetry within the mouse 3' IgH regulatory region includes two virtually identical enhancers (Calpha3'E and hs3). J. Immunol. 158 (10): 4780-7.
5. Shrivastava, A., Saleque, S., Kalpana, G.V., Artandi, S., Goff, S. P. and Calame, K. 1993. Inhibition of transcriptional regulator Yin-yang 1 by association with c-myc. Science. 262(5141): 1889-92.
1. Submitted as a SCORE (SC1) grant to NIH in January 2009.
Grant Title: “Genetic and epigenetic regulation of hematopoiesis”.
Amount requested $1,900,000 (total costs). Requested period: 12/01/2009-11/30/2014. Received a priority score of 19 (July 2009). Pending award notification.
2. Submitted to NSF in July 2009.
Grant Title: “Genetic and epigenetic programming of hematopoietic cell fates”.
Amount requested $855,000. Requested period: 08/01/2010-07/31/2013..
1. Graduate rotation student :1- Ayisha Sookdeo.
2. Undergraduate students: 3- Khris Persaud, Justin Kauffman and Godwin Ogundare.
Mentoring: Currently mentoring and training 3 undergraduate students (2 minority) in the laboratory.
Major advising: Currently advising 12 Biology Major students (5 women, 3 underserved minority, 8 other minority) on career goals, research opportunities and academic development.